The much-awaited Padma Bridge was opened for vehicular movement at 6am on Sunday.
Hundreds of vehicles were seen waiting to cross the multipurpose bridge.
A total of fourteen booths are being used to collect tolls in manual system.
A motorcyclist will have to pay Tk100 to cross the bridge, while the toll is Tk2,400 for a large bus with three axles and Tk2,800 for a medium truck.
The rate has been set at Tk1,600 for a mini truck (up to five tonnes); Tk2,100 for a medium truck (5-8 tonnes); Tk2,800 for a medium truck (8-11 tonnes); Tk5,500 for truck (up to three axle) and Tk6,000 for trailer (up to four axles).
An additional Tk1,500 will be charged for each axle for a trailer larger than four axles.
Prime Minister Sheikh Hasina unveiled the plaque of the 6.15 kilometre Padma Bridge at Mawa end in Munsiganj at a colourful ceremony on Saturday noon.
The prime minister became the first person to cross the Padma Bridge by paying toll.