China's President Xi Jinping has warned that foreign powers will "get their heads bashed" if they attempt to bully or influence the country.
He delivered a defiant speech at an event marking the centenary of the ruling Communist Party on Thursday, reports BBC.
Mr Xi also said Beijing would not allow "sanctimonious preaching", in remarks widely seen as directed at the US.
It comes as China faces criticism over alleged human rights abuses and its crackdown in Hong Kong.
Relations between the US and China have worsened in recent times over trade, espionage and the pandemic.
The issue of Taiwan is also a major source of tension. While democratic Taiwan sees itself as a sovereign state, Beijing views the island as a breakaway province.
The US, under its own laws, is required to provide Taiwan with the means to defend itself should Beijing use force to take the island back.
On Thursday Mr Xi said China maintains an "unshakeable commitment" to unification with Taiwan.
"No one should underestimate the resolve, the will and ability of the Chinese people to defend their national sovereignty and territorial integrity," he said.
The 100th anniversary celebrations on Thursday morning saw military jet fly-pasts, cannon salutes and patriotic songs played.
A carefully vetted crowd were in attendance in Beijing's Tiananmen Square, many of whom were not wearing masks.
The country has seen a media blitz in recent weeks promoting a party-approved version of China's history.
Hong Kong is also marking its handover anniversary on the same day.
What stood out in Xi's message?
Mr Xi, who spoke for around an hour, reiterated the role of the party in modern China, saying that it has been central to the country's growth and that attempts to separate it from the people would "fail".
"Only socialism can save China, and only socialism with Chinese characteristics can develop China," he said.
He added that "we will never allow anyone to bully, oppress or subjugate China".
"Anyone who dares try to do that will have their heads bashed bloody against the Great Wall of Steel forged by over 1.4 billion Chinese people," he said.
China has repeatedly accused the US of trying to curb its growth - and these comments are also seen as a reference to Washington.
On Hong Kong and Macau - which he said both retain a "high degree of autonomy" - they should "accurately implement the principles of 'One Country, Two Systems".
How did China prepare for this anniversary?
The Chinese Communist Party (CCP), which was founded in 1921, came to power 72 years ago after a long civil war.
In that time the country has undergone massive changes - but some of these milestones were conspicuously missing in the propaganda drive.
On Monday, an art performance titled The Great Journey was staged at the Bird's Nest stadium in Beijing, where performers put on extravagant set-pieces detailing the history of the party and country.
But significant events such the Cultural Revolution purges, the 1989 Tiananmen Square protests, and the pro-democracy protests in Hong Kong were missing, reported AFP news agency.
Since April, Chinese cinemas were ordered to screen propaganda films, known as "red films", at least twice a week.
A song, called 100%, that praised China's achievements and featured 100 rappers was also released.
"Red tourism" has also become popular, with travel companies such as Ctrip launching 100 unique routes for "red pilgrims".
But not all were pleased with the propaganda.
"Now when I turn on the TV at night, dozens of stations are airing dramas about revolutionary wars," a Beijing resident told BBC Chinese.