The US has announced sanctions against Russia in response to what it says are cyber-attacks and other hostile acts.
The measures, which target dozens of Russian entities and officials, aim to deter "Russia's harmful foreign activities", the White House said.
The statement says Russian intelligence was behind last year's massive "SolarWinds" hack, and accuses Moscow of interference in the 2020 election.
Russia denies all the allegations and says it will respond in kind.
The sanctions announced on Thursday are detailed in an executive order signed by President Joe Biden. They come at a tense time for relations between the two countries.
Last month the US targeted seven Russian officials and more than a dozen government entities over the poisoning of Kremlin critic Alexei Navalny. Russia says it was not involved.
In a call with Russian President Vladimir Putin on Tuesday, Mr Biden vowed to defend US national interests "firmly", while proposing a meeting with Mr Putin to find areas where the two countries could work together.
According to Thursday's White House statement, the new sanctions show the US "will impose costs in a strategic and economically impactful manner on Russia" if it continues its "destabilizing international action".
It reaffirms the administration's view that the Russian government is behind cyber-attacks and has been trying to "undermine the conduct of free and fair democratic elections" in the US and allied nations.
It specifically blames the Russia's foreign intelligence service, the SVR, for the SolarWinds attack, which gave cyber-criminals access to 18,000 government and private computer networks.
Last December then US Secretary of State Mike Pompeo said he believed Russia was behind the SolarWinds attack.
The latest sanctions target 32 entities and officials accused of trying to influence the 2020 US presidential election "and other acts of disinformation".
Ten diplomats, including alleged spies, are being expelled from the US. The executive order also bars US financial institutions from purchasing rouble-denominated bonds from June.
Shortly after the sanctions were announced, Russia's foreign ministry called them "hostile steps which dangerously raise the temperature of confrontation".
"Such aggressive conduct will of course get a decisive response," the statement added.
The US ambassador has been called to the foreign ministry.
The EU, Nato and the UK have all issued statements in support of the US measures.
In his first foreign policy speech in February, Mr Biden made it clear he planned to stand up to Russia. "The days of the United States rolling over in the face of Russia's aggressive actions… are over," he said.
The statement was in a stark contrast to the stance adopted by Mr Biden's predecessor, Donald Trump, who rarely criticised Mr Putin.
In a report last month, US intelligence agencies concluded the Russian president had probably directed online efforts to help Donald Trump win a second term as US president.
The US has also warned Russia against aggressive actions in Ukraine. Russia is beefing up its military presence in the border area.
Source: BBC